Hamamatsu Education in Digital Imaging

Welcome to the Hamamatsu educational website featuring a wide variety of review articles, interactive tutorials, image galleries, and product descriptions designed to assist our visitors in exploring the fascinating world of digital imaging. Highlighted this month is the new ImagEM, a brand new member of the C9100 electron multiplying CCD camera series. Combining our decades of experience with high vacuum technology and all the latest advances in CCD circuit design this new camera offers application independent imaging. From the very brightest high dynamic range applications to single photon imaging over long integrations, this camera provides images with the very best signal to noise ratios. The key to this versatility is the E2V CCD97 CCD and Hamamatsu creative engineering. New methods for driving this well known CCD offer special modes of operation for fast, low noise operation and special patent pending synchronization with spinning disk confocal and multi-point confocal microscopy.

Featured Image

Indian Muntjac Deer Skin Fibroblast Cells

The culture of Indian Muntjac deer skin fibroblast cells presented in the digital image above was labeled for the cytoskeletal filamentous actin network with Alexa Fluor 350 conjugated to phalloidin, and for the cell nucleus with SYTOX Green. Additionally, cellular mitochondria were stained with MitoTracker Red CMXRos, a complex aminated xanthene derivative. Images were recorded in grayscale with a Hamamatsu Orca-AG camera system coupled to an Olympus BX-51 microscope equipped with bandpass emission fluorescence filter optical blocks provided by Semrock. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.